In vivo imaging is largely a problem of scale. The difference between healthy cells and disease is often molecules measured in nanometers, but the organs these cells make up are measured in centimeters. Molecular imaging solves this problem of imaging over five orders of magnitude by using a two-part approach: molecular probes interact with targets on the nanometer scale and provide a tag for the wide field-of-view imager. We have developed imagers for molecular imaging based on fluorescence lifetime and up-conversion based probes developed at the Center for Molecular Imaging Research (CMIR) at Massachusetts General Hospital (MGH).
C. Salthouse, S. Hildebrand, R. Weissleder, and U. Mahmood, "Design and Demonstration of a Small-Animal Up-Conversion Imager" Optics Express, vol. 16, no. 26, pp. 21731-21737, December 2008.
C. Salthouse, R. Upadhyay, R. Weissleder, and U. Mahmood, "In Vivo Discrimination of Two Far Red Dyes with Identical Absorption and Emission Spectra Based on Fluorescent Lifetime for Improved Multiplexing" presented at the Radiological Society of North America November 2007.
C. Salthouse, R. Upadhyay, R. Weissleder, and U. Mahmood, "Fluorescent Lifetime Discrimination of Activatable Probe Signal from Autofluorescence in Whole Mouse Imaging" presented at the Joint Society for Molecular Image / Academy of Molecular Imaging Conference September 2007.