Lensless Fluorescence Imaging

Fluorescence microscopy has revolutionized medicine and biological science with its ability to study the behavior and chemical expressions of living cells. Fluorescent probes can label cell components or cells of a particular type. Clinically the impact of fluorescence imaging can be seen in the diagnosis of cancers, AIDS, and other blood related disorders. Although fluorescence imaging devices have been established as a vital tool in medicine, the size, cost, and complexity of fluorescence microscopes limits their use to central laboratories. The work described in this thesis overcomes these limitations by developing a low cost integrated fluorescence microscope so single use fluorescence microscopy assays can be developed. These assays will enable at-home testing, diagnostics in resource limited settings, and improved emergency medicine.

Relevant Publications

C.D.Salthouse, A.Shanmugam, 'Lensless Fluorescence Imaging Systems to Measure Surface Sample Flow', 225th Electro Chemical Society Meeting, 2014

A.Shanmugam, C.D.Salthouse, 'Microfluidic on CMOS with laser Cut Adhesive Tape', 225th Electro Chemical Society Meeting, 2014

A.Shanmugam, C.D.Salthouse, 'Lensless Fluorescence Imaging with Height Calculation', Journal of Biomedical Optics, 19(1):016002, 2013

A.Shanmugam, C.D.Salthouse, 'Miniaturized On-Sensor Fluorescence Flow Cytometer', IEEE Sensors, pp. 1-4, Oct 2012

A.Shanmugam, C.D.Salthouse, '3D Lensless Fluorescence Imaging', I2MTC, pp. 1109-1112 May 2012

A.Shanmugam, K.Okiah, C.D.Salthouse, 'Lensless Fluorescence Imaging of Flowing Beads in Microfluidics', The Electrochemical Society, 2012