Medical Diagnostics

Flow cytometers and fluorescence microscopes can detect small differences in fluorescence signals from healthy and diseased cells to detect disease, but because these techniques are looking at individual cells they are poorly suited to screening large samples for rare cells such as circulating tumor cells or cancer stem cells. Bulk measurements can find rare cells, but are plagued by high background signals. We have demonstrated that ability to separate probe signal from autofluorescence using fluorescence lifetime.

Relevant Papers

C. Salthouse, R. Upadhyay, R. Weissleder, and U. Mahmood, "Fluorescent Lifetime Discrimination of Activatable Probe Signal from Autofluorescence in Whole Mouse Imaging" presented at the Joint Society for Molecular Image / Academy of Molecular Imaging Conference September 2007.